001You all please join me in the pledge of allegiance. Pledge of allegiance. The flag. >> All right. Want to welcome everyone to this June the 1st meeting of the board of school trustees for the Metropolitan School District of Mount Vernon. Uh we will start u with the recognition of delegations. Persons wishing to be recognized to speak on any agenda item or to be heard as part of a section of the agenda dealing with delegation or citizen comments or to identify themselves by name and the point on the agenda where they wish to be recognized. Right. Anyone else? All right, Lois, we have that noted and we have a presentation by the FFA. You just get better. because that just helps prepare Wait a minute. Okay. or older or not really. So that's like the blood
002test they use to detect the disorder, but it's not expensive enough to catch really small amount minuscule amounts of the hormone. Um, and it's it is not advanced to detect the the disorder early on. So my goal was to improve it. So I was going to I 11 strategies um and I tested all of them in our ratio testing. So in basically it has a gene to it. It's called a tetrome. So what happens is it's able to connect to more AC molecules so that you can detect more of really small amounts of the ail. Um, so that also um doing this is before I conducted the experiment at Western Kentucky university but before that I had to purchase all the reagents and the supplies and then it wasn't so the reagents um we had
003to use protection antibodies protein G's biotin a wash buffer and SA so you could reduce the background noises then besides that we had to use so what we went about doing had 20 horses right so 10 the horses was the first group they were under the age of 15 and then the other group is older so they're above the age of 15 um but we use ports um and the other so you take it's called a butterfly needle so you insert it in the horse's jugular vein attach um the it's a tube um and then it automat the tube automatically fills up with blood. Um, EDTA tube is basically standard in most testing situations. The EDTA is called an anti-coagulant. So, the anti-coagulant would mix in with the blood and preserve it. So, because the
004hormone is super fragile and we are on a time constraint for the project um and for the sample itself, we had four hours and after that four hours we wouldn't be able to use the sample. So, I took those samples and I sent it into and I collected the plasma. So, I pipeted out the plasma into my centrifuge tubes and then flash froze them in liquid nitrogen nitrogen and then I put them in our freezer which was at -20° um um so long term. So after we had that uh we he and both went down Kentucky um and there are two phases to the project itself. So the first the first phase is the ratio testing. I had to find the best ratio that would work in the blood samples because if I used too
005much of a detection antibbody, it wouldn't it would give me false readings and if it gave me too little um it would be too quiet of a signal. So in this um experiment we use what's called a sandwich amino essay. So what happens is the antibodies on top of each other and they lock down on top of the like that's the molecular so we use a so clea is chemical lime amino acid um so it doesn't turn the ACT into a different color it just turns it into light basically and then I would take that and I would put it in a machine and the machine would be able to tell me how much light is produced is called RLU values. Um the machine is not going to tell me how much ACT is in
006that blood sample. The machine is going to tell me how much light is produced. That is why I have ACT standards. So that's kind of the most important part of this project. That's kind of what helped us with the data. Um the ACT standard was our known amount of the hormone. Um, so then when you have known amount of hormone and you compare it against the blood samples, we don't know how much ACT is in it. We're not able to test that. A machine won't do that for us. So that's why you make the graph that has all the standards on it and then you can compare them to so you can figure out the confidence in it. So um, the ratio testing you take your 96 well plate which is in this little picture.
007really tiny, but it has 96 little wells in it. And you put in your standards and then your ratios. And then you put that and the one that worked best was the 1 to2 molar ratio. That ratio and I used it in the blood sample process. that process. The second phase of it, I took the blood samples uh with the standards and with uh the ratio um and we tested the young all 20 horses, the young horses and the older horses. So from there after I was able to do that then you would want to do an analysis on it. So you have your you'd have to calculate your coefficient of coefficient of variation, your LLD, your LLQ um and then you'd have your raw L RLU signals. So coefficient of variation is just um
008it's a percentage of the variability in the tray or in the plate and you would just turn that as a percentage. And then the LOD stands for lower limit of detection. So the lower limit of detection is basically it's able to detect it and how well it's going to be able to detect it. And then the LOQ stands for lower limit of quantification. And then that's going to give us an accurate measurement of what's happening inside the wells. So after that then you go on to your uh the date. So if you look at the gray line, this line right under here, um that's the original that's the original test that dockers are using for PP. Then the blue line is my new test and it's more steeper and it's more. So that showed us
009that reduction in the LO here. This one, this is going to show your LOD and your L. So all it's showing is that this one getting more slope. So saying at 100 uh pic per milliliter, you're able to see this much of the concentration or the LRL. So um I improved it by 29.7 reduction in a lower limit detection and 34.7% um in LQ compared to the standard the one the blood test that they used but they improved it this whole method could be applied to towards more cancer biomarkers and they could base off this because AC is a biomarker Um, so I hope a doctor can pick it up and then they can go on their own clinical trial and maybe it could be used for cancer and detecting it early, detecting cancer and
010other tumors early on. >> Very neat. >> Okay, you guys have any questions? Cut it really short because I was >> Yes. So, um, we just go straight to state for the aggra science fair. >> Yeah. Um, >> and then next week Yeah, >> we just go to Nolesville, Indiana. >> Yeah, it's an Indianapolis. >> Me, too. Me, too. Um, so basically I knew I wanted to do another research project this year and I had connections to there's a professor at uh Middle Tennessee State University. Um, and I told her I need I want to do another research project. I really really enjoy this and I need all of your knowledge on horses because she was the ecoin professor and she gave me a ton of tips and she helped me um kind of create
011this but she said you need to just go after you need to look at what you're most interested in. Go to read as many veterary journals as you can. That's exactly what I did and I knew I was interested into the cancer part because I thought that was I just thought that was interesting. So >> no, >> yeah, we did u the efficacy of calling self. was taught. Yes, I was a great help. >> Any other questions? >> Did you call the statistical analysis on that? >> I'm sorry. Can you say that again? I'd say did you do all the statistical analysis the deviations and your okay? >> Well, uh something like that. I'd like to go into research ecoin research. Um a lot of my research uh >> Yeah. Um so started I use
012our school's equipment. So we have a centrifuge, we have pipets. Um and then we had to purchase a lot of lot of Yeah. Yeah. Yeah. But we had to buy I had to buy all of it. It came from some wild places. The [laughter] but >> really well that's cool. But uh yeah, so we went to Western Kentucky University to do this, but it was it was a struggle because the original plan was to do it at UE because they had the equipment and then they backed out on us because the machine broke. So then I talked to um like the animal testing facility, middle run, Astroenica. I mean I I searched high and low everywhere. I was on playing ABC and then I got to LHS feel like we went to to we were
013going to go to Purdue and do it but um it was way too far but Western Kentucky had what we needed so we were able to get the project done >> my I'm a sophomore I I'm a sophomore I'm going into my junior Awesome. >> A couple more. I'll let I'll let I'm Louis Island. I'm a recent graduate of Outer High School overview real fast before we get into it. They diversified farming. So they grow grain crops and then they feed them to their cows. So diversified the livestock system. Most of the grain that is uh grown is fed to the cattle and the extra is sold out. Value added agriculture. Since we are able to walk the grain off the farm by meaning feeding our cattle, we are able to say efficient business structure.
014We aren't uh this business is not in a deficit for assets. Uh so it's not it's efficient with it assets and not over overly um doesn't have too much assets to pay off. So it's not businesses. It's in the four uh business are companies uh the equipment they own all the equipment uh for all the grain and infrastructure and moldy roast the crops uh and this just shows how the business flows that cattle and moldy grain own nothing but the crops and those all the land from Uh, but as most farms, the moldy grain doesn't it doesn't own all this land. So, it still goes to other landlord uh landlords and tenants to be able to in corn and soybean production and to the cost. So that the cost and also on the other category
015I made bushels just goes to show that the first 60 corn Oh, great. But go along. Little over a million dollars. There you go. Where's it going? sheets here. We baked the balance sheets to show that the business structure why it's kind of done to school different people that are in the farm will own the equipment. So all the fat cattle back cattle is 1.7 million Thank you all for your time. >> So, do you guys have any questions? >> This is legit a legit business you're running. >> No, no, no. It's like a hypothetical business, but >> hypothetical, >> but we put the we put the numbers to it. So, it's like those are realistic numbers. You could do it if you want if you >> we often like nothing USDA county averages stuff
016like that. >> So it looks like uh you were using cash rent. Do you see much in thirds twoirds the way farmers split in more or is it all cash? Well, for this for this project, we did more cash rent shrimp, it's easier to put the number two uh third share crop, it's kind of harder, but then also with the uh beef cattle operation, >> um that's also more difficult. But everything that the beef cow fed, even the bedding, all came from the the moldy grain. >> Yeah, but those are all fake businesses. We just made them up and put them together. >> I didn't want to underestimate you guys. >> But they are they are >> I [laughter] know. I like that name, too. Anybody else have any questions? >> Great. >> Thank you.
017All right, thank you very much for the presentations. Very inspiring stuff. All right, we'll move into the meeting portion. We have Mr. Williams joining us by uh video conference. Uh so we will be engaged in a roll call vote tonight and we will move into recommended actions. >> Hey uh is it okay to go ahead and take A and B together? >> Yes it is. And we have provided you with the minutes from the May 18th meeting. And also we have vouchers um checks through 41070 and uh 147846 through 14797 and electronic transfers ET00003440 through ET3448. I know this is normally your part that you read and I guess guess I just decided I'd go ahead and do it today for you, but we're we're recommending [laughter] >> both of these actions tonight. >> All
018right, >> we will take up A and B. Uh is there a motion to approve the minutes from the May 18th, 2026 meeting as prepared or as correction? Is there a motion to authorize payments of payroll checks through 410070 voucher checks 147846 through 147907 and electronic transfers ET00003440 through ET00003448 as recommended. voided payroll and voucher checks not listed on the voucher listings in the above sequences are not proof or approved for payment by this action. >> So moved. >> Second motion by Messi, second by Mr. Isac. Any comments, questions or concerns? Seeing none is Mr. Williams, can you hear us? Okay. Okay. All right. We will move to a roll call vote. Miss Pessic. >> Yes. >> Miss Butler? >> Yes. >> Mr. Isaac? >> Yes. >> I vote yes. Uh Mr. Williams and those
019those mo those measures pass. U have no unfinished business. So we'll move to new business. >> Uh we have the following resignations. Fletcher Cook, English teacher at the high school and yearbook sponsor. Brett Gail, custodian at West Elementary. and Kenda Hensley, elementary teacher at Mars Elementary School. >> Okay. Is there a motion to accept the resignations effective immediately unless otherwise indicated from the list as read by Dr. Stewart? >> Second. >> A motion by Mr. Isaac, seconded by Miss Butler. Any comments, questions, or concerns? Seeing none, we'll move to the roll call vote. Mr. Cesi, >> yes. >> Miss Butler? >> Yes. >> Mr. Isaac? >> Yes. >> I vote yes. Mr. Williams. All right. And then we have some employment recommendations. >> We do. Amber Nation for in information systems manager, Macy Potter, elementary
020teacher at West Elementary, Morgan Rieger, assistant coach for volleyball at the junior high, Natasha Schmidzer, assistant coach, girls soccer at the high school, Miranda Winesapple, elementary teacher at West, and Amy Winger, math teacher at the junior high. >> Right. Very good. Is there a motion to confirm the employment from the list as read by Dr. Ster? Who moves? >> And we're going to give that to Miss Butler. Mr. Williams, you're a second. >> Any comments, questions, or concerns? >> Seeing none, we'll move to the roll call vote. Mr. Cesi, >> yes. >> Meller, >> yes. >> Mr. >> Yes. >> I vote yes. Mr. Williams. Motion carries. >> And we have some meetings we need to approve. Audrey McGee will attend the uh CS and AIPD next week in Bloomington, Indiana on June 14th through
021the 19th, 2026. And Lindsay Schmidt is to attend the Secretary's Professional Learning Network in Jasper, Indiana on July 30th, 2026. Is there a motion to adopt a resolution permitting personnel listed to attend educational meetings? >> So move. >> Second. >> Motion is made by Mr. Isaac, seconded by Miss Passi. Any comments, questions, or concerns? >> Seeing none, we'll move to a roll call vote. Mr. Pessi, >> yes. >> Mr. Butler, >> yes. >> Mr. Isaac, >> yes. >> I vote yes. Mr. Williams. >> All right. Then a motion. Um, and then we have a summer some summer camps or a summer camp. >> We have enclosed uh the information regarding a planned summer skills camp for 2026. Coaches have been conducting camps for several years. They are responsible for conducting the camp as well as
022for setting and collecting fees. Advertising of the camp etc. The fee collected by the presenters goes directly to them and from these fees they pay for fees that they pay for expenses plus salaries involved. The district does not charge for the use of facilities. >> All right. Is there a motion to approve the use of facilities for summer camps for 2026 as recommended? >> So moved. >> Second. Miss Butler, second by Mr. Isaac. Uh, and then the dates will have >> Yes, Kathy. Those are July 10, June 10th. Is that correct? For the soccer. >> Yeah, that starts at >> 15 to 18. Sorry. >> That's fine. We We'll see them. Okay. Any other comments or questions? Think that we'll move the roll call vote. Mr. Vessic. >> Yes. Miss Buller. >> Yes. >> Mr.
023Isaac, >> yes. >> I vote yes. Mr. Williams. >> And the motion uh carries. All right. Um [clears throat] we've reached the uh point on the agenda where we have comments or questions from delegations or citizens. Lois That was cool. Okay, >> good. >> Oh, great. Maybe just a little teaser maybe. Yeah, they're okay. Yeah. >> Right. >> Sure. That's just that's great. Yeah. Um, I haven't been out there in a a few weeks. Have you been out there, Alyssa? I don't think so. No, we discussed that, but I think that's pushing it too fast. So, I think he's going to wait until closer to the fall. It's we >> we purchased it. We are still working through our new building projects and just getting everything lined up with the architectural company and the attorneys
024and we really don't have anything new to report on that. Um we're just moving through that process and uh initial initial plans for that. So we will be communicating about that regularly though. We're going to move on. We'll have some um Yeah, we'll have uh it it was a senior prank. That's that's what I'll say. And nobody was hurt. Nothing was damaged. >> No, not at all. Might have been a little bit of cleanup, but there wasn't any nothing that cost any money to clean up or repair. There were not drugs stolen. Well, that that is all rumor. Yeah. No, we were good. Nothing. It was just a mess. That's what we'll say. That's right. I might be camping out here next year on that night. But no, I'm not doing that. >> Yeah. No,
025I think we're gonna beef up beef up our alarm systems. >> Yeah. So, >> Oh, yeah. Yeah, >> but we're moving on. [laughter] >> We're glad to provide some entertainment. [laughter] >> Anyone else? Anything else? Paula, right? Um I don't think there's anything from non staff communications. Okay. from the superintendent. >> Uh just that our cafeteria supply bids are in and we are processing through that and as soon as we know more uh we will report out on that. >> All right. Any other questions from board members? >> Nope. We'll move to future business. If you will go ahead and take us on out, please. >> Sure. So, we'll be back here June 15th at same time, 5:30 p.m. and then July 6th. Um our office is closed on the 3rd. Is that correct, Kathy?
026So, the AO will be closed on July 3rd, just in case anybody wanted to come visit us that day. Um, and then we'll be back here on July 20th again. Um, and actually after that, we have a couple policies um, special education policies that we are in uh, going to introduce to the board and bring back um, the at the next meeting. Um, but they're just standard policies that we need to make sure we have in place. So, >> we're working on some handbook updates. We are working on employee handbook and this is the time of year we really can look through our policies and make sure that they're up to date and they uh are in compliance with the different uh subsembs. All right. And we stand at Jerus.